Molecular diagnostics

The rapidSTRIPE detection system is developed as a new diagnostic platform for very fast, efficient and specific detection of microbial pathogens, like bacteria, protozoa or viruses. The modular structure of this system combines all steps of molecular diagnostics – isolation of nucleic acids, amplification and detection of target nucleic acids.

 

bs_Rickettsien_Set_2

AJbs_H1N1_rapidSTRIPE_Assay_e

For detection of Influenca suptype H1N1. The H1N1 Assay contains all reagents for isolation of viral RNA, amplification and detection of H1N1. The isolation of viral RNA from swab samples is based on an optimized method for isolation of viral nucleic acids (patend pending). The final amplification and detection is based on the RAH technology (Rapid Amplification Hybridization) and take place on a simple lateral flow stripe.

Latest press release: Analytik Jena Launches H1N1 Rapid Test

 

 
Order number
 Amount
845-IV-1000010 10 reactions
845-IV-1000025 25 reactions
845-IV-1000050 50 reactions
 

For the detection of successful bacterial DNA isolation from foodstuff after cultivation using a stomacher. The test is based on the amplification of a general microbial specific target sequence and the subsequent gelelectrophoretically analysis. The test avoid false negative results referring to test for the detection of pathogens due to an insufficient DNA quality.

 

Application menthod:
The isolation of the nucleic acids has to be done using the rapidSTRIPE Food DNA Extraction Kit. The protocols inside the accordant user manual have to be followed exactly. The operation of the test was optimized by using nucleic acids, which were isolated by the above mentioned extraction kits. Alternatively, also nucleic acids, which were isolated by other methods could be used.

Kit components:
Well Strip, Food DNA positive control, Primer, dNTP Mix, Polymerase, PCR Buffer, PCR-grade H2O

Order-No.
 Amount
845-IS-2007010 10 reactions
845-IS-2007100 100 reactions
 

For the specific detection of Listeria monocytogenes from nucleic acids isolated from foodstuff after cultivation using rapidPCR and a novel stripe assay.

 

Test description and principle
The rapidSTRIPE Listeria m. Stripe Assay detects the nucleic acids of the pathogens directly inside the sample material. The test contains 3 modules, which functions are optimized to each other. This kit contains modul 2 and 3.

Kit components
Well Strip, L. monocytogenes positive control, Primer, dNTP mix, Polymerase, PCR Buffer, PCR-grade-H2O, Lateral Flow Dipsticks, Running Buffer, Sample Tubes

Order-No.
 Amount
845-IS-1005010 10 reactions
845-IS-1005100 100 reactions
 

For the specific detection of Salmonella enteritica from nucleic acids isolated from foodstuff after cultivation using rapidPCR and a novel stripe assay.

 

Test description and principle
The rapidSTRIPE Salmonella e. Stripe Assay detects the nucleic acids of the pathogens directly inside the sample material. The test contains 3 modules, which functions are optimized to each other. This kit contains modul 2 and 3.

Kit components
Well Strip, S. enterica positive control, Primer, dNTP mix, Polymerase, PCR Buffer, PCR-grade H2O, Lateral Flow Dipsticks, Running Buffer, Sample Tubes

Order-No.
 Amount
845-IS-1004010 10 reactions
845-IS-1004100 100 reactions
 

For the extremely fast isolation of bacterial DNA from foodstuff after standard cultivation steps using a stomacher.

 

Storage conditions:
The rapidSTRIPE Food DNA Extraction Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K and the MAG Suspension at 4°C!Store dissolved Proteinase K at –20°C. Repeated freezing and thawing will reduce the activity dramatically.Dividing of the Proteinase K into aliquots and storage at –20°C is recommended.

Kit components:
Lysis Tube, Buffer, Lysis Solution, Binding Solution, Proteinase K, Washing Solution, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-IS-3200010 10 reactions
845-IS-3200100 100 reactions
 

For detection of Babesia infections from nucleic acids isolated from ticks using rapidPCR and a novel stripe assay.

 

Test description and principle
The rapidSTRIPE Babesia Stripe Assay detects the nucleic acids of the pathogens directly inside the sample material (ticks). The test contains 3 modules, which functions are optimized to each other. This kit contains modul 2 and 3.

Performance assessment, spectrum of application and specificity
his test was used for the analysis of about 400 ticks to determine the occurrence of Babesia infections. Positive test results were sequenced consecutively to verify the results and to classify Babesia. The following tick species were analysed:

  • Dermacentor reticulatus
  • Ixodes hexagonus
  • Haemaphysalis concinna
  • Ixodes ricinus

Results from ticks may not be adducted as exclusive basis for further therapies!

Kit components
Well Strip, Babesia positive control, Primer, dNTP mix, Polymerase, PCR Buffer, PCR-grade H2O, Lateral Flow Dipsticks, Running Buffer, Sample Tubes

Order-No.
 Amount
845-IS-1009010 10 reactions
845-IS-1009100 100 reactions
 

For the detection of successful DNA isolation from ticks based on the amplification of a tick specific target sequence and subsequent gelelectrophoretically analysis. The test avoid false negative results referring to the test for the detection of pathogens due to a insufficient DNA quality.

 

Application methods:
The isolation of the nucleic acids has to be done using the rapidSTRIPE Food DNA Extraction Kit. The protocols inside the accordant user manual have to be followed exactly. The operation of the test was optimized by using nucleic acids, which were isolated by the above mentioned extraction kits. Alternatively, also nucleic acids, which were isolated by other methods could be used.

Kit components:
Well Strip, Tick DNA positive control, Primer, dNTP mix, Polymerase, PCR Buffer, PCR-grade H2O

Order-No.
 Amount
845-IS-2006010 10 reactions
845-IS-2006100 100 reactions
 

For the detection of Ehrlichia infections from nucleic acids isolated from ticks using rapidPCR and a novel stripe assay. This test is can be used for the detection of Anaplasma phagocytophilum incl. related Ehrlichia.

 

Test description and principle
The rapidSTRIPE Anaplasma p. Stripe Assay detects the nucleic acids of the pathogens directly inside the sample material (ticks). The test contains 3 modules, which functions are optimized to each other. This kit contains modul 2 and 3.

Performance assessment, spectrum of application and specificity
This test was used for the analysis of about 400 ticks in the ambit of Berlin to determine the occurrence of Anaplasma phagocytophilum infections. Positive test results were sequenced consecutively to verify the results. The following tick species were analysed:

  • Dermacentor reticulatus
  • Ixodes hexagonus
  • Haemaphysalis concinna
  • Ixodes ricinus

Results from ticks may not be adducted as exclusive basis for further therapies!

Kit components
Well Strip, Anaplasma p. positive control, Primer, dNTP mix, Polymerase, PCR Buffer, PCR-grad H2O, Lateral Flow Dipsticks, Running Buffer, Sample Tubes

Order-No.
 Amount
845-IS-1007010 10 reactions
845-IS-1007100 100 reactions
 

For the detection of Borrelia infections from nucleic acids isolated from ticks using rapidPCR and a novel stripe assay.

 

Test description and principle
The rapidSTRIPE Borrelia Stripe Assay detects the nucleic acids of the pathogens directly inside the sample material (ticks). The test contains of 3 modules, which functions are optimized to each other. This kit contains modul 2 and 3.

Performance assessment, spectrum of application and specificity
This test was used for the analysis of about 400 ticks to determine the occurrence of Borrelia infections. Positive test results were sequenced consecutively to verify the results and to classify Borrelia. The following tick species were analysed:

  • Dermacentor reticulatus
  • Ixodes hexagonus
  • Haemaphysalis concinna
  • Ixodes ricinus

Results from ticks may not be adducted as exclusive basis for further therapies!

Kit components
Well Strip, Borrelia positive control, Primer, dNTP mix, Polymerase, PCR Buffer, PCR-grade H2O, Lateral Flow Dipsticks, Running Buffer, Sample Tubes

Order-No.
 Amount
845-IS-1002010 10 reactions
845-IS-1002100 100 reactions
 

For the detection of Rickettsia infections from nucleic acids isolated from ticks using rapidPCR and a novel stripe assay.

 

Test description and principle
The rapidSTRIPE Rickettsia Stripe Assay detects the nucleic acids of the pathogens directly inside the sample material (ticks). The test contains 3 modules, which functions are optimized to each other. This kit contains modul 2 and 3.

Performance assessment, spectrum of application and specificity
This test was used for the analysis of about 400 ticks to determine the occurrence of Rickettsia infections. Positive test results were sequenced consecutively to verify the results and to classify Rickettsia. The following tick species were analysed:

  • Dermacentor reticulatus
  • Ixodes hexagonus
  • Haemaphysalis concinna
  • Ixodes ricinus

Results from ticks may not be adducted as exclusive basis for further therapies!

Kit components
Well Strip, Rickettsia positive control, Primer, dNTP mix, Polymerase, PCR Buffer, PCR-grade H2O, Lateral Flow Dipsticks, Sample Tubes

Order-No.
 Amount
845-IS-1001010 10 reactions
845-IS-1001100 100 reactions
 

For the extremely fast and simultaneously isolation of DNA and RNA from ticks using SpeedMill P12 or other beads based homogenizer. RNA viruses (e.g. FSME) can be determined next to the detection of bacterial pathogens.

 

Storage conditions:
The rapidSTRIPE Tick DNA/RNA Extraction Kit should be stored dry, at room temperature
(14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Lysis Tube, Lysis Solution, Washing Solutions, RNAse-free Water, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-IS-5100010 10 reactions
845-IS-5100100 100 reactions
 

For the extremely fast isolation of DNA from ticks using SpeedMill P12 or other beads based homogenizer. The purified DNA is also dedicated for the further detection of bacteria and protozoa from the tick tissue.

 

Storage conditions:
The rapidSTRIPE TICK DNA Extraction Kit should be stored dry, at room temperature
(14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Lysis Tube, Lysis Solution, Binding Solution, Precipitation Buffer, Proteinase K, Washing Solutions, Elution Buffer, Pre-Filter, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-IS-3100010 10 reactions
845-IS-3100100 100 reactions
 

Rapid Test for detection of B.pertussis infections in human. The test is based on isolation of DNA form nasopharyncs specimen (swabs) an subsequent detection of b.pertussis DNA using the RAH technology (Rapid Amplification Hybridization). The detection take place on a lateral flow test stripe. The complete procedure  is finished within 1.5 h. The test is certified for in vitro diagnostics.

 

Test description and principle
The rapidSTRIPE Pertussis Stripe Assay detects the nucleic acids of the pathogens directly inside the sample material. The test contains 3 modules, which functions are optimized to each other. This kit contains modul 2 and 3.

Kit components:
Well Strip, B. pertussis Positivkontrolle, Primer, dNTP Mix, Polymerase, PCR Buffer, PCR-grade H2O, Lateral Flow Dipsticks Running Buffer, Sample Tubes

Order-No.
 Amount
845-IV-1100010 10 reactions
845-IV-1100100 100 reactions